Chapter Name: Reproduction in Plants
Activity Name: Tumbler in Reproduction in Plants
This experiment aims to observe the process of budding in yeast cells using a simple setup involving a glass tumbler, water, sugar, and yeast powder.
- Glass tumbler
- Yeast powder
- Glass slides
- Cover slips
Step by Step Procedure:
- Take a glass tumbler and fill it with some water.
- Add a tablespoon of sugar and half a spoon of yeast powder into the tumbler containing water.
- Mix the contents well until the sugar and yeast powder are dissolved.
- Cover the glass tumbler with a lid and leave it undisturbed for a day to allow the yeast to undergo budding.
- After a day, take a glass slide and place a small drop of the yeast solution on it.
- Cover the yeast solution with a cover slip to create a slide for microscopic observation.
- Examine the slide under a microscope.
Under the microscope, you will be able to observe budding yeast cells. These cells will appear as small, spherical structures with smaller “buds” growing on their surfaces, resembling water bubbles with new bubbles forming on them.
- Handle the glass tumbler and microscope carefully to avoid any accidents.
- Use clean glass slides and cover slips to ensure accurate observations.
- Make sure to cover the tumbler during the incubation period to prevent contamination.
- Take appropriate safety measures while using yeast powder to avoid inhalation or contact with eyes or skin.
Lesson Learnt from Experiment:
This experiment demonstrates a simple method to observe the process of budding in yeast cells. Budding is a form of asexual reproduction in yeast, where new daughter cells grow as small outgrowths from the parent cell. It helps students understand the basic principles of reproduction in unicellular organisms and the concept of asexual reproduction.