Chapter Name: Plants: Parts and functions
Activity Name: Stomata Observation in Plants: Parts and functions
Observing stomata on a leaf using a microscope.
- Cover slips
Step by Step Procedure:
- Peel off the outer layer of a leaf carefully. You can use a sharp blade or forceps to gently remove the outer layer. Make sure to handle the leaf with care to avoid damaging it.
- Place the peeled leaf section on a clean microscope slide.
- Add a small drop of water onto the leaf section. The water helps in making the observation clearer.
- Take another microscope slide with a cover slip and gently place it over the leaf section. Ensure that the cover slip is not pressing too hard on the leaf.
- Carefully transfer the slide to the stage of the microscope.
- Start with the lowest magnification on the microscope and focus on the leaf section.
- Gradually increase the magnification until you can clearly see the details of the leaf surface.
- Look for bean-shaped structures on the leaf surface. These are the stomata. They appear as small openings or pores on the leaf.
- Compare your observation with a diagram or figure depicting stomata to confirm their presence.
Under the microscope, you will observe:
- The peeled leaf section with a layer of water on it.
- Numerous small bean-shaped structures on the leaf surface.
- The stomata appear as openings or pores on the leaf.
- Handle the leaf section and microscope slides carefully to avoid damaging them.
- Use a clean microscope slide and cover slip to prevent any interference with the observation.
- Do not use excessive pressure while placing the cover slip to avoid crushing the leaf section.
- Be cautious while using sharp tools like blades or forceps and ensure proper safety measures.
Lesson Learnt from Experiment:
Through this experiment, you will learn that stomata are small openings present on the surface of leaves. They resemble bean-shaped structures and play a vital role in the exchange of gases between the plant and the atmosphere.
Stomata allow the plant to take in carbon dioxide for photosynthesis and release oxygen and water vapor. This experiment helps in understanding the structure and function of stomata, which are crucial for plant survival.